A biophysical and structural study of two chitinases from Agave tequilana and their potential role as defense proteins.

Sierra-Gómez Y, Rodríguez-Hernández A, Cano-Sánchez P, Gómez-Velasco H, Hernández-Santoyo A, Siliqi D, Rodríguez-Romero A
FEBS J (2019 Jul 10)
PMID: 31291689
doi: 10.1111/febs.14993
Submitted to SASBDB: 2018 May 28
Published in SASBDB:

SASDDE7 – Class I chitinase 1 from Agave tequilana

Chitinase 1 experimental SAS data
DAMMIF model
Sample: Chitinase 1 monomer, 32 kDa Agave tequilana protein
Buffer: MES 50 mM, pH: 6
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2017 Apr 26
RgGuinier 2.6 nm
Dmax 9.7 nm
VolumePorod 52 nm3

SASDDA6 – Class I chitinase 2 from Agave tequilana

Chitinase 2 experimental SAS data
DAMMIF model
Sample: Chitinase 2 monomer, 32 kDa Agave tequilana protein
Buffer: MES 50 mM, pH: 6
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2017 Apr 19
RgGuinier 2.4 nm
Dmax 9.8 nm
VolumePorod 49 nm3