Integrin α6β4 Recognition of a Linear Motif of Bullous Pemphigoid Antigen BP230 Controls Its Recruitment to Hemidesmosomes.

Manso JA, Gómez-Hernández M, Carabias A, Alonso-García N, García-Rubio I, Kreft M, Sonnenberg A, de Pereda JM
Structure 27(6):952-964.e6 (2019 Jun 4)

PMID: 31006587 doi: 10.1016/j.str.2019.03.016

Submitted to SASBDB: 2018 Jun 7 Published in SASBDB: 2019 Mar 14

SASDDE8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4) Sample: Integrin beta-4 fragment with final part of the connecting segment and the third and fourth FnIII domains monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 37 nm3

SASDDF8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463E Sample: Integrin beta-4 (1436-1666) R1463E monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.3 nm Dmax 9.5 nm VolumePorod 35 nm3

SASDDG8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1463A Sample: Integrin beta-4 (1436-1666) R1463A monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 36 nm3

SASDDH8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468R Sample: Integrin beta-4 (1436-1666) A1468R monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.8 nm Dmax 10.9 nm VolumePorod 35 nm3

SASDDJ8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant A1468D Sample: Integrin beta-4 (1436-1666) A1468D monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.7 nm Dmax 11.0 nm VolumePorod 34 nm3

SASDDK8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475E Sample: Integrin beta-4 (1436-1666) R1475E monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.6 nm Dmax 9.5 nm VolumePorod 42 nm3

SASDDL8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1475A Sample: Integrin beta-4 (1436-1666) R1475A monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 35 nm3

SASDDM8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542E Sample: Integrin beta-4 (1436-1666) R1542E monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.3 nm Dmax 9.5 nm VolumePorod 35 nm3

SASDDN8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant R1542A Sample: Integrin beta-4 (1436-1666) R1542A monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.3 nm Dmax 8.5 nm VolumePorod 34 nm3

SASDDP8 – Integrin beta4, fragment of the cytoplasmic region that includes the final part of the connecting segment and the third and fourth FnIII domains (FnIII-3,4). Point mutant T1663R Sample: Integrin beta-4 (1436-1666) T1663R monomer, 26 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 26 RgGuinier 2.4 nm Dmax 9.5 nm VolumePorod 35 nm3