Synthetic FRET biosensor Twitch 2B

Pablo Trigo Mourino Submitted to SASBDB: 2018 May 25
Published in SASBDB:

SASDD97 – Synthetic FRET biosensor Twitch 2B Ca-bound

Synthetic FRET biosensor Twitch 2B experimental SAS data
Synthetic FRET biosensor Twitch 2B Kratky plot
Sample: Synthetic FRET biosensor Twitch 2B monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 5 mM CaCl2, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 17
RgGuinier 2.7 nm

SASDDA7 – Synthetic FRET biosensor Twitch 2B Ca-free

Synthetic FRET biosensor Twitch 2B experimental SAS data
Synthetic FRET biosensor Twitch 2B Kratky plot
Sample: Synthetic FRET biosensor Twitch 2B monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 10 mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2015 Mar 17
RgGuinier 4.1 nm

SASDDB7 – Synthetic FRET biosensor Twitch 2B N532F Ca-bound

Synthetic FRET biosensor Twitch2B N532F experimental SAS data
Synthetic FRET biosensor Twitch2B N532F Kratky plot
Sample: Synthetic FRET biosensor Twitch2B N532F monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 5 mM CaCl2, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 30
RgGuinier 2.8 nm

SASDDC7 – Synthetic FRET biosensor Twitch 2B N532F Ca-free

Synthetic FRET biosensor Twitch2B N532F experimental SAS data
Synthetic FRET biosensor Twitch2B N532F Kratky plot
Sample: Synthetic FRET biosensor Twitch2B N532F monomer, 62 kDa synthetic construct protein
Buffer: 20 mM MOPS 100 mM NaCl 10 mM EDTA, pH: 7
Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 30
RgGuinier 3.9 nm