Biophysical insights from a single chain camelid antibody directed against the Disrupted-in-Schizophrenia 1 protein.

Yerabham ASK, Müller-Schiffmann A, Ziehm T, Stadler A, Köber S, Indurkhya X, Marreiros R, Trossbach SV, Bradshaw NJ, Prikulis I, Willbold D, Weiergräber OH, Korth C
PLoS One 13(1):e0191162 (2018)

PMID: 29324815 doi: 10.1371/journal.pone.0191162

Submitted to SASBDB: 2018 May 16 Published in SASBDB: 2018 Jun 5

SASDDK7 – Disrupted-in-Schizophrenia 1 protein (DISC1 12d2) Sample: Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 monomer, 19 kDa protein Buffer: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Oct 1 RgGuinier 2.6 nm Dmax 7.3 nm VolumePorod 41 nm3

SASDDL7 – anti-DISC1 single-domain camelid antibody VHH B5 Sample: Anti-DISC1 single-domain camelid antibody VHH B5 monomer, 14 kDa protein Buffer: 25 mM Tris, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 19 RgGuinier 1.8 nm Dmax 8.4 nm VolumePorod 21 nm3

SASDDM7 – Complex of disrupted-in-Schizophrenia 1 protein (DISC1 12D2) and anti-DISC1 single-domain camelid antibody VHH B5 Sample: Anti-DISC1 single-domain camelid antibody VHH B5 monomer, 14 kDa protein Disrupted- in-schizophrenia 1 (DISC1 12D2) 691-836 monomer, 19 kDa protein Buffer: 25 mM Tris-HCl, 150 mM NaCl, 1mM DTT, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2016 Feb 19 RgGuinier 3.1 nm Dmax 10.4 nm VolumePorod 77 nm3