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Standard proteins
Darja Ruskule
Published in SASBDB:
2015 Jul 7
SASDA32
– Bovine serum albumin in HEPES
Sample:
Bovine Serum Albumin monomer, 69 kDa
Bos taurus (Bovine)
protein
Buffer:
50 mM HEPES 50 mM KCl, pH: 7.5
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 12
R
g
Guinier
2.9
nm
D
max
9.3
nm
Volume
Porod
97
nm
3
SASDA52
– Alcohol dehydrogenase in PBS
Sample:
Alcohol dehydrogenase 1 tetramer, 147 kDa
Saccharomyces cerevisiae
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 20
R
g
Guinier
3.2
nm
D
max
8.9
nm
Volume
Porod
178
nm
3
SASDA62
– bAmylase in PBS
Sample:
Beta-amylase tetramer, 224 kDa
Ipomoea batatas
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 20
R
g
Guinier
4.2
nm
D
max
12.7
nm
Volume
Porod
214
nm
3
SASDA82
– Apoferritin in PBS and glycerol
Sample:
Ferritin light chain 24-mer, 479 kDa
Equus caballus
protein
Buffer:
PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 20
R
g
Guinier
6.8
nm
D
max
12.8
nm
Volume
Porod
619
nm
3
SASDA92
– Catalase in PBS
Sample:
Catalase tetramer, 240 kDa
Bos taurus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 17
R
g
Guinier
4.1
nm
D
max
10.9
nm
Volume
Porod
296
nm
3
SASDAA2
– Conalbumin in PBS
Sample:
Ovotransferrin monomer, 78 kDa
Gallus gallus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 21
R
g
Guinier
3.8
nm
D
max
11.0
nm
Volume
Porod
117
nm
3
SASDAB2
– Cytochrome c from equine heart
Sample:
Cytochrome c monomer, 12 kDa
Equus caballus
protein
Heme C monomer, 1 kDa
Buffer:
25 mM HEPES, 100 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment:
SAXS data collected at EMBL P12, PETRA III
on 2018 Apr 8
R
g
Guinier
1.3
nm
D
max
3.7
nm
Volume
Porod
12
nm
3
SASDAC2
– Lysozyme in sodium acetate
Sample:
Lysozyme C monomer, 14 kDa
Gallus gallus
protein
Buffer:
40 mM Sodium Acetate, pH: 3.8
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 21
R
g
Guinier
1.5
nm
D
max
4.0
nm
Volume
Porod
17
nm
3
SASDAG2
– Lysozyme in sodium acetate
Sample:
Lysozyme C monomer, 14 kDa
Gallus gallus
protein
Buffer:
40 mM Sodium Acetate, pH: 3.8
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 21
R
g
Guinier
1.5
nm
D
max
4.0
nm
Volume
Porod
21
nm
3
SASDAH2
– Myoglobin in PBS
Sample:
Myoglobin monomer, 17 kDa
Equus caballus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 20
R
g
Guinier
1.7
nm
D
max
5.0
nm
Volume
Porod
32
nm
3
SASDAK2
– Myoglobin in PBS
Sample:
Myoglobin monomer, 17 kDa
Equus caballus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 20
R
g
Guinier
1.6
nm
D
max
5.0
nm
Volume
Porod
32
nm
3
SASDAL2
– Ovalbumin in PBS
Sample:
Ovalbumin monomer, 43 kDa
Gallus gallus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 18
R
g
Guinier
2.5
nm
D
max
7.8
nm
Volume
Porod
74
nm
3
SASDAN2
– Bovine pancreatic ribonuclease A in PBS (WAXS)
Sample:
Ribonuclease pancreatic monomer, 16 kDa
Bos taurus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 18
R
g
Guinier
1.6
nm
D
max
5.0
nm
Volume
Porod
15
nm
3
SASDAQ2
– Ubiquitin in sodium acetate
Sample:
Ubiquitin monomer, 9 kDa
Bos taurus
protein
Buffer:
40 mM Sodium acetate 150 mM NaCl, pH: 5.5
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 18
R
g
Guinier
1.3
nm
D
max
4.9
nm
Volume
Porod
12
nm
3
SASDAR2
– Bovine pancreatic ribonuclease A in PBS
Sample:
Ribonuclease pancreatic monomer, 16 kDa
Bos taurus
protein
Buffer:
PBS, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Sep 18
R
g
Guinier
1.6
nm
D
max
5.0
nm
Volume
Porod
17
nm
3
SASDAK6
– Glucose Isomerase
Sample:
Xylose isomerase tetramer, 172 kDa
Streptomyces rubiginosus
protein
Buffer:
PBS, 50% Glycerol, 0.076 M NaCl, pH: 7.4
Experiment:
SAXS data collected at EMBL X33, DORIS III, DESY
on 2012 Apr 24
R
g
Guinier
3.4
nm
D
max
9.7
nm
Volume
Porod
293
nm
3