FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.

Grinter R, Hay ID, Song J, Wang J, Teng D, Dhanesakaran V, Wilksch JJ, Davies MR, Littler D, Beckham SA, Henderson IR, Strugnell RA, Dougan G, Lithgow T
PLoS Biol 16(8):e2006026 (2018 Aug)

PMID: 30071011 doi: 10.1371/journal.pbio.2006026

Submitted to SASBDB: 2018 May 19 Published in SASBDB: 2018 Jun 13

SASDDQ6 – The ferredoxin protease, FusC Sample: Ferredoxin Protease monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Buffer: 20 mM Tris, 150 mM NaCl, 0.03 % NaN3, 5.0 % glycerol, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6 RgGuinier 3.7 nm Dmax 12.8 nm VolumePorod 152 nm3

SASDDR6 – The ferredoxin protease, FusC, E83A mutant Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6 RgGuinier 3.7 nm Dmax 12.7 nm VolumePorod 152 nm3

SASDDS6 – The ferredoxin protease, FusC, with Arabidopsis ferredoxin (co-SEC-SAXS) Sample: Ferredoxin Protease monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6 RgGuinier 3.7 nm Dmax 13.2 nm VolumePorod 156 nm3

SASDDT6 – The ferredoxin protease, FusC, E83A mutant with Arabidopsis ferredoxin (co-SEC-SAXS) Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaN3, 5% glycerol, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Apr 6 RgGuinier 3.6 nm Dmax 12.7 nm VolumePorod 161 nm3

SASDDU6 – The ferredoxin protease, FusC, E83A mutant Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 13.8 nm VolumePorod 152 nm3

SASDDV6 – The ferredoxin protease, FusC, E83A mutant + 3 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 13.8 nm VolumePorod 150 nm3

SASDDW6 – The ferredoxin protease, FusC, E83A mutant + 5 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 13.7 nm VolumePorod 148 nm3

SASDDX6 – The ferredoxin protease, FusC, E83A mutant + 10 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 13.6 nm VolumePorod 149 nm3

SASDDY6 – The ferredoxin protease, FusC, E83A mutant + 20 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 13.6 nm VolumePorod 155 nm3

SASDDZ6 – The ferredoxin protease, FusC, E83A mutant + 30 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 14.0 nm VolumePorod 156 nm3

SASDD27 – The ferredoxin protease, FusC, E83A mutant + 50 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.8 nm Dmax 14.0 nm VolumePorod 160 nm3

SASDD37 – The ferredoxin protease, FusC, E83A mutant + 70 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 14.0 nm VolumePorod 165 nm3

SASDD47 – The ferredoxin protease, FusC, E83A mutant + 100 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 13.7 nm VolumePorod 177 nm3

SASDD57 – The ferredoxin protease, FusC, E83A mutant + 150 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.8 nm Dmax 14.2 nm VolumePorod 178 nm3

SASDD67 – The ferredoxin protease, FusC, E83A mutant + 200 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 14.0 nm VolumePorod 175 nm3

SASDD77 – The ferredoxin protease, FusC, E83A mutant + 300 µM Arabidopsis ferredoxin Sample: Ferredoxin protease E83A mutant monomer, 101 kDa Pectobacterium atrosepticum SCRI1043 protein Arabidopsis ferredoxin 2 monomer, 11 kDa Arabidopsis thaliana protein Buffer: 20 mM Tris, 150 mM NaCl, pH: 7.8 Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2017 Oct 26 RgGuinier 3.7 nm Dmax 14.0 nm VolumePorod 180 nm3