Dynamics in a pure encounter complex of two proteins studied by solution scattering and paramagnetic NMR spectroscopy.

Xu X, Reinle W, Hannemann F, Konarev PV, Svergun DI, Bernhardt R, Ubbink M
J Am Chem Soc 130(20):6395-403 (2008 May 21)

PMID: 18439013 doi: 10.1021/ja7101357

Published in SASBDB: 2015 Jul 7

SASDAN5 – Cross-linked complex CytC_Adr Sample: Cytochrome C monomer, 11 kDa Escherichia coli protein Adrenodoxin monomer, 11 kDa Escherichia coli protein Buffer: 20 mM HEPES 2 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jun 15 RgGuinier 2.1 nm Dmax 8.5 nm VolumePorod 42 nm3

SASDAP5 – Native complex CytC_Adr Sample: Cytochrome C dimer dimer, 22 kDa Escherichia coli protein Adrenodoxin dimer dimer, 22 kDa Escherichia coli protein Buffer: 20 mM HEPES 2 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Jun 15 RgGuinier 2.9 nm Dmax 9.5 nm VolumePorod 64 nm3