OCP-FRP protein complex topologies suggest a mechanism for controlling high light tolerance in cyanobacteria.

Sluchanko NN, Slonimskiy YB, Shirshin EA, Moldenhauer M, Friedrich T, Maksimov EG
Nat Commun 9(1):3869 (2018 Sep 24)

PMID: 30250028 doi: 10.1038/s41467-018-06195-0

Submitted to SASBDB: 2018 Jul 4 Published in SASBDB: 2018 Aug 7

SASDDE9 – Synechocystis fluorescence recovery protein FRPcc in the pre-oxidized state (CC mutant) Sample: Fluorescence recovery protein dimer, 28 kDa Synechocystis sp. PCC … protein Buffer: 20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1 RgGuinier 2.9 nm Dmax 13.0 nm VolumePorod 43 nm3

SASDDF9 – Synechocystis orange carotenoid-binding protein devoid of the NTE (OCP-ΔNTE, orange form) Sample: Orange carotenoid-binding protein monomer, 34 kDa Synechocystis sp. PCC … protein Buffer: 20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1 RgGuinier 2.2 nm Dmax 7.4 nm VolumePorod 57 nm3

SASDDG9 – The 2:1 complex of Synechocystis disulphide-trapped Fluorescence recovery protein dimer (CC mutant) and orange carotenoid-binding protein-ΔNTE (orange form) Sample: Fluorescence recovery protein dimer, 28 kDa Synechocystis sp. PCC … protein Orange carotenoid-binding protein monomer, 34 kDa Synechocystis sp. PCC … protein Buffer: 20 mM Tris, 150 mM NaCl, 3% glycerol, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 1 RgGuinier 3.0 nm Dmax 13.0 nm VolumePorod 102 nm3