Structural analysis of monomeric retroviral reverse transcriptase in complex with an RNA/DNA hybrid.

Nowak E, Potrzebowski W, Konarev PV, Rausch JW, Bona MK, Svergun DI, Bujnicki JM, Le Grice SF, Nowotny M
Nucleic Acids Res 41(6):3874-87 (2013 Apr 1)
PMID: 23382176
doi: 10.1093/nar/gkt053 		Published in SASBDB:

SASDAV5 – apo XMRV RT

apo XMRV RT experimental SAS data
CRYSOL model
Sample: Apo XMRV RT monomer, 75 kDa Escherichia coli protein
Buffer: 10 mM HEPES 100 mM KCl 5% Glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 8
RgGuinier 4.0 nm
Dmax 13.5 nm
VolumePorod 160 nm3

SASDAW5 – XMRV RT + DNA/RNA hybrid

apo XMRV RTRNA_DNA hybrid substrate experimental SAS data
CRYSOL model
Sample: Apo XMRV RT monomer, 75 kDa Escherichia coli protein
RNA_DNA hybrid substrate monomer, 15 kDa
Buffer: 10 mM HEPES 100 mM KCl 5% Glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 8
RgGuinier 3.5 nm
Dmax 11.5 nm
VolumePorod 155 nm3