Membranes prime the RapGEF EPAC1 to transduce cAMP signaling

Sartre C, Peurois F, Ley M, Kryszke M, Zhang W, Courilleau D, Fischmeister R, Ambroise Y, Zeghouf M, Cianferani S, Ferrandez Y, Cherfils J
Nature Communications 14(1) (2023 Jul 12)

doi: 10.1038/s41467-023-39894-4

Submitted to SASBDB: 2022 Nov 21 Published in SASBDB: 2023 Jul 14

SASDQA8 – Rap guanine nucleotide exchange factor Epac1 Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4 RgGuinier 3.2 nm Dmax 10.7 nm VolumePorod 130 nm3

SASDQB8 – Rap guanine nucleotide exchange factor Epac1 with cAMP Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4 RgGuinier 3.5 nm Dmax 13.0 nm VolumePorod 140 nm3

SASDQC8 – Rap guanine nucleotide exchange factor Epac1 with CE3F4 inhibitor Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4 RgGuinier 3.3 nm Dmax 11.4 nm VolumePorod 140 nm3

SASDQD8 – Rap guanine nucleotide exchange factor Epac1 with cAMP and CE3F4 inhibitor Sample: Rap guanine nucleotide exchange factor 3 monomer, 82 kDa Homo sapiens protein Buffer: 20 mM Tris pH 8.0, 150 mM NaCl, 0.5 mM EDTA, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Oct 4 RgGuinier 3.3 nm Dmax 11.8 nm VolumePorod 134 nm3