Structural Organization and Dynamics of Homodimeric Cytohesin Family Arf GTPase Exchange Factors in Solution and on Membranes.

Das S, Malaby AW, Nawrotek A, Zhang W, Zeghouf M, Maslen S, Skehel M, Chakravarthy S, Irving TC, Bilsel O, Cherfils J, Lambright DG
Structure (2019 Oct 5)

PMID: 31601460 doi: 10.1016/j.str.2019.09.007

Submitted to SASBDB: 2019 Jan 17 Published in SASBDB: 2019 Oct 14

SASDEV9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO truncation mutant Sample: Cytohesin-2; ARNO truncation mutant monomer, 40 kDa Homo sapiens protein Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2015 Nov 25 RgGuinier 2.7 nm Dmax 9.9 nm VolumePorod 63 nm3

SASDEW9 – Cytohesin-2; ARF nucleotide-binding site opener, ARNO Sample: Cytohesin-2 ARF nucleotide-binding site opener dimer, 93 kDa Homo sapiens protein Buffer: 300 mM NaCl, 2 mM 2-mercaptoethanol and 30 mM Tris-HCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jun 23 RgGuinier 4.8 nm Dmax 19.7 nm VolumePorod 145 nm3