Structure and Dynamics of a Promiscuous Xanthan Lyase from Paenibacillus nanensis and the Design of Variants with Increased Stability and Activity.

Jensen PF, Kadziola A, Comamala G, Segura DR, Anderson L, Poulsen JN, Rasmussen KK, Agarwal S, Sainathan RK, Monrad RN, Svendsen A, Nielsen JE, Lo Leggio L, Rand KD
Cell Chem Biol 26(2):191-202.e6 (2019 Feb 21)

PMID: 30503284 doi: 10.1016/j.chembiol.2018.10.016

Submitted to SASBDB: 2018 Aug 17 Published in SASBDB: 2018 Dec 6

SASDEF3 – Paenibacillus xanthan lyase (PXL) at 4 °C Sample: Paenibacillus xanthan lyase monomer, 113 kDa Paenibacillus sp-62047 protein Buffer: 20 mM Tris,, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 15 RgGuinier 3.7 nm Dmax 13.1 nm VolumePorod 137 nm3

SASDEG3 – Paenibacillus xanthan lyase (PXL) at 20 °C Sample: Paenibacillus xanthan lyase monomer, 113 kDa Paenibacillus sp-62047 protein Buffer: 20 mM Tris,, pH: 8.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 15 RgGuinier 3.8 nm Dmax 13.8 nm VolumePorod 134 nm3