The crystal structure of netrin-1 in complex with DCC reveals the bifunctionality of netrin-1 as a guidance cue.

Finci LI, Krüger N, Sun X, Zhang J, Chegkazi M, Wu Y, Schenk G, Mertens HDT, Svergun DI, Zhang Y, Wang JH, Meijers R
Neuron 83(4):839-849 (2014 Aug 20)

PMID: 25123307 doi: 10.1016/j.neuron.2014.07.010

Published in SASBDB: 2015 Jul 7

SASDAZ5 – NetrinVIV Sample: Netrin-1 monomer, 49 kDa Homo sapiens protein Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26 RgGuinier 3.9 nm Dmax 13.5 nm VolumePorod 100 nm3

SASDA26 – DCC56 Sample: Deleted in Colorectal Cancer (FN5 & FN6) monomer, 26 kDa Homo sapiens protein Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26 RgGuinier 3.2 nm Dmax 11.0 nm VolumePorod 38 nm3

SASDA76 – NetrinVIV DCC56 complex Sample: Netrin-1 monomer, 49 kDa Homo sapiens protein Deleted in Colorectal Cancer (FN5 & FN6) monomer, 26 kDa Homo sapiens protein Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31 RgGuinier 5.1 nm Dmax 17.0 nm VolumePorod 120 nm3

SASDA86 – NetrinVIV DCC56(M933R) complex Sample: Netrin-1 monomer, 49 kDa Homo sapiens protein Deleted in Colorectal Cancer (FN5 & FN6) M933R mutant monomer, 26 kDa Homo sapiens protein Buffer: 25 MES mM 200 mM NaCl 50 mM Tris 0.2 M ammonium sulfate (NH4)2(SO4) 1mM calcium chloride CaCl2, pH: 7 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 26 RgGuinier 4.0 nm Dmax 14.0 nm VolumePorod 95 nm3