Quantitative live-cell imaging and 3D modeling reveal critical functional features in the cytosolic complex of phagocyte NADPH oxidase.

Ziegler CS, Bouchab L, Tramier M, Durand D, Fieschi F, Dupré-Crochet S, Mérola F, Nüße O, Erard M
J Biol Chem (2019 Jan 10)

PMID: 30630949 doi: 10.1074/jbc.RA118.006864

Submitted to SASBDB: 2018 Aug 28 Published in SASBDB: 2019 Jan 16

SASDEJ3 – Truncated neutophil cytosol factor 1, p47phox [1-342] Sample: Neutophil cytosol factor 1 monomer, 40 kDa Homo sapiens protein Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2009 Oct 16 RgGuinier 2.6 nm Dmax 10.0 nm VolumePorod 58 nm3

SASDEK3 – The neutrophil cytosol factor 1 (p47phox) subunit of phagocyte NADPH oxidase Sample: Neutrophil cytosol factor 1 monomer, 46 kDa Homo sapiens protein Buffer: 50 mM HEPES, 100 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2008 Apr 23 RgGuinier 3.2 nm Dmax 12.5 nm VolumePorod 77 nm3

SASDEL3 – The neutrophil cytosol factor 2 (p67phox) subunit of phagocyte NADPH oxidase Sample: Neutrophil cytosol factor 2 monomer, 61 kDa Homo sapiens protein Buffer: 20 mM HEPES, 50 mM NaCl, 1 mM EDTA, 2 mM DTT, 5% glycerol, pH: 8 Experiment: SAXS data collected at D24, LURE on 2003 Apr 9 RgGuinier 4.3 nm Dmax 16.0 nm VolumePorod 113 nm3