Molecular basis of histone tail recognition by human TIP5 PHD finger and bromodomain of the chromatin remodeling complex NoRC.

Tallant C, Valentini E, Fedorov O, Overvoorde L, Ferguson FM, Filippakopoulos P, Svergun DI, Knapp S, Ciulli A
Structure 23(1):80-92 (2015 Jan 6)
PMID: 25533489
doi: 10.1016/j.str.2014.10.017
Published in SASBDB:

SASDA46 – TIP5 in Tris

Bromodomain adjacent to zinc finger domain protein 2A experimental SAS data
BUNCH model
Sample: Bromodomain adjacent to zinc finger domain protein 2A monomer, 27 kDa Homo sapiens protein
Buffer: 20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Mar 16
RgGuinier 3.0 nm
Dmax 10.0 nm
VolumePorod 45 nm3

SASDA56 – BAZ2B in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminal experimental SAS data
EOM/RANCH model
Sample: Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa Homo sapiens protein
Buffer: 20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 24
RgGuinier 4.0 nm
Dmax 14.5 nm
VolumePorod 43 nm3

SASDA66 – BAZ2B-18mer complex in Tris

Bromodomain adjacent to zinc finger domain protein 2B, C-terminalH3Kac9Kac14 experimental SAS data
Bromodomain adjacent to zinc finger domain protein 2B, C-terminal H3Kac9Kac14 Kratky plot
Sample: Bromodomain adjacent to zinc finger domain protein 2B, C-terminal monomer, 28 kDa Homo sapiens protein
H3Kac9Kac14 monomer, 5 kDa Homo sapiens protein
Buffer: 20 mM Tris 500 mM NaCl 2 mM DTT 10 microM ZnCl2, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2012 Sep 24
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 52 nm3