Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument.

Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
J Appl Crystallogr 51(Pt 6):1623-1632 (2018 Dec 1)

PMID: 30546289 doi: 10.1107/S1600576718014462

Submitted to SASBDB: 2018 Sep 29 Published in SASBDB: 2018 Nov 11

SASDEY4 – Ribonuclease A monomer from in-house SEC-SAXS Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23 RgGuinier 1.5 nm Dmax 4.8 nm VolumePorod 10 nm3

SASDEZ4 – Carbonic Anhydrase from in-house SEC-SAXS Sample: Carbonic anhydrase 2 monomer, 29 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24 RgGuinier 1.9 nm Dmax 5.3 nm VolumePorod 38 nm3

SASDE25 – Human insulin hexamer from in-house SEC-SAXS Sample: Human insulin hexamer, 35 kDa Homo sapiens protein Buffer: 7 mM sodium phosphate, 60 mM phenol, 200 µM Zn(CH3COO)2, 23 mM NaCl, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 26 RgGuinier 2.0 nm Dmax 5.9 nm VolumePorod 39 nm3

SASDE35 – Ovalbumin monomer from in-house SEC-SAXS Sample: Ovalbumin monomer, 43 kDa Gallus gallus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24 RgGuinier 2.4 nm Dmax 7.8 nm VolumePorod 55 nm3

SASDE45 – Conalbumin monomer from in-house SEC-SAXS Sample: Ovotransferrin monomer, 76 kDa Gallus gallus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24 RgGuinier 3.0 nm Dmax 10.1 nm VolumePorod 100 nm3

SASDE55 – Apoferritin monomer from in-house SEC-SAXS Sample: Ferritin light chain 24-mer, 476 kDa Equus caballus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 24 RgGuinier 5.1 nm Dmax 12.3 nm VolumePorod 685 nm3

SASDE65 – Bovine serum albumin monomer from in-house SEC-SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23 RgGuinier 2.8 nm Dmax 9.2 nm VolumePorod 92 nm3

SASDE75 – Bovine serum albumin monomer from in-house SEC-SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23 RgGuinier 2.8 nm Dmax 9.9 nm VolumePorod 104 nm3

SASDE85 – Bovine serum albumin monomer from in-house SEC-SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23 RgGuinier 2.8 nm Dmax 8.2 nm VolumePorod 85 nm3

SASDE95 – Bovine serum albumin monomer from in-house SEC-SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23 RgGuinier 2.9 nm Dmax 8.6 nm VolumePorod 74 nm3

SASDEA5 – Bovine serum albumin monomer from in-house SEC-SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 25 RgGuinier 2.8 nm Dmax 9.6 nm VolumePorod 78 nm3

SASDEB5 – Bovine serum albumin from in-house SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: 50 mM HEPES, pH: 7.5 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 May 4 RgGuinier 2.8 nm Dmax 9.4 nm VolumePorod 99 nm3

SASDEC5 – Bovine serum albumin dimer from in-house SEC-SAXS Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein Buffer: PBS, pH: 7.4 Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23 RgGuinier 4.1 nm Dmax 13.8 nm VolumePorod 200 nm3

SASDED5 – Bovine serum albumin monomer from synchrotron SEC-SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: PBS + 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 14 RgGuinier 2.7 nm Dmax 8.7 nm VolumePorod 84 nm3

SASDEE5 – Bovine serum albumin from synchrotron SAXS Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein Buffer: 50 mM HEPES, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26 RgGuinier 2.8 nm Dmax 9.5 nm VolumePorod 85 nm3