Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase.

Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
Sci Rep 8(1):18008 (2018 Dec 20)

PMID: 30573755 doi: 10.1038/s41598-018-36195-5

Submitted to SASBDB: 2018 Oct 3 Published in SASBDB: 2019 Jan 16

SASDEG5 – apo human adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13 RgGuinier 3.7 nm VolumePorod 300 nm3

SASDEH5 – AMP/fumarate-bound human adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.6 nm VolumePorod 256 nm3

SASDEJ5 – AICAR/fumarate-bound human adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.6 nm VolumePorod 250 nm3

SASDEK5 – AMP-bound human adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM AMP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.7 nm VolumePorod 292 nm3

SASDEL5 – AICAR-bound human adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.7 nm VolumePorod 291 nm3

SASDEM5 – apo neanderthal adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13 RgGuinier 3.7 nm VolumePorod 304 nm3

SASDEN5 – AMP/fumarate-bound neanderthal adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.7 nm VolumePorod 258 nm3

SASDEP5 – AMP-bound neanderthal adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM AMP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.7 nm VolumePorod 294 nm3

SASDEQ5 – AICAR-bound neanderthal adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.7 nm VolumePorod 292 nm3

SASDER5 – AICAR/fumarate-bound neanderthal adenylosuccinate lyase (ADSL) Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24 RgGuinier 3.6 nm VolumePorod 252 nm3