Structural basis for activation of plasma-membrane Ca²⁺-ATPase by calmodulin.

Nitsche J, Josts I, Heidemann J, Mertens HD, Maric S, Moulin M, Haertlein M, Busch S, Forsyth VT, Svergun DI, Uetrecht C, Tidow H
Commun Biol 1:206 (2018)

PMID: 30511020 doi: 10.1038/s42003-018-0203-7

Published in SASBDB: 2018 Dec 3

SASDES4 – ACA8 in stealth nanodisc (SANS, 100% D2O) Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5 Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28 RgGuinier 4.0 nm Dmax 13.0 nm VolumePorod 202 nm3

SASDET4 – ACA8 complex with Calmodulin (75% deuterated) in stealth nanodisc (SANS, 100% D2O) Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Calmodulin-7 (deuterated 75%) monomer, Arabidopsis thaliana protein Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5 Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28 RgGuinier 4.3 nm Dmax 15.0 nm VolumePorod 217 nm3

SASDEU4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc (SANS, 100% D2O) Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Calmodulin-7 monomer, Arabidopsis thaliana protein Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5 Experiment: SANS data collected at SANS-1, Heinz Maier-Leibnitz Zentrum on 2017 Aug 28 RgGuinier 4.8 nm Dmax 18.0 nm VolumePorod 297 nm3

SASDEV4 – ACA8 protein (apo) in stealth nanodisc Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8 RgGuinier 5.3 nm Dmax 20.0 nm VolumePorod 626 nm3

SASDEW4 – ACA8 complex with Calmodulin (hydrogenated) in stealth nanodisc Sample: Membrane scaffold protein 1D1 (deuterated, 75%) dimer, 49 kDa protein 1-palmitoyl-2-palmitoleoyl-sn-glycero-3-phosphocholine (deuteration: 78% head, 92% acyl), 1 kDa Escherichia coli Calcium-transporting ATPase 8, plasma membrane-type monomer, 118 kDa Arabidopsis thaliana protein Calmodulin-7 monomer, Arabidopsis thaliana protein Buffer: 30 mM Tris, 150 mM NaCl, 1mM MgCl2, 1 mM CaCl2, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 8 RgGuinier 5.9 nm Dmax 22.0 nm VolumePorod 805 nm3