Solution structures of long-acting insulin analogues and their complexes with albumin.

Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019 Mar 1)

PMID: 30950398 doi: 10.1107/S2059798318017552

Submitted to SASBDB: 2018 Oct 3 Published in SASBDB: 2019 Apr 7

SASDEV5 – Insulin detemir tri-hexamer Sample: Insulin detemir 18-mer, 106 kDa protein Buffer: 5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH: 7.4 Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25 RgGuinier 3.3 nm Dmax 11.3 nm VolumePorod 137 nm3

SASDEW5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site II Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4 Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25 RgGuinier 3.5 nm Dmax 13.0 nm VolumePorod 162 nm3

SASDEX5 – Albumin-insulin detemir 1:6 complex, binding in Südlow's Site I Sample: Insulin detemir (Levemir(R), Novo Nordisk A/S) hexamer, 35 kDa protein Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein Buffer: 6.9 mM Na2HPO4, 11.9 mM m-cresol, 13.7 mM phenol, 157.3 mM glycerol, 38.5 mM NaCl, pH: 7.4 Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25 RgGuinier 3.5 nm Dmax 13.0 nm VolumePorod 162 nm3

SASDEZ5 – Albumin-insulin detemir 2:12 complex, P2 symmetry Sample: Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein Buffer: 8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4 Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25 RgGuinier 5.4 nm Dmax 19.5 nm VolumePorod 309 nm3

SASDEY5 – Albumin-insulin detemir 2:12 complex, P1 symmetry Sample: Human Albumin (Recombumin(R) Alpha, Albumedix Ltd.) monomer, 66 kDa protein Insulin detemir (Levemir(R), Novo Nordisk A/S) dodecamer, 71 kDa protein Buffer: 8.8 mM Na2HPO4, 10.6 mM m-cresol, 12.2 mM phenol, 140.9 mM glycerol, 56.9 mM NaCl, pH: 7.4 Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25 RgGuinier 5.4 nm Dmax 19.5 nm VolumePorod 309 nm3

SASDE26 – Albumin-insulin degludec 1:12 complex Sample: Human Albumin (Recombumin(R) Elite, Albumedix Ltd.) monomer, 66 kDa protein Insulin degludec(Tresiba(R), Novo Nordisk A/S) dodecamer, 73 kDa protein Buffer: 25 mM Na2HPO4, 15.9 mM m-cresol, 15.9 mM phenol, 212.8 mM glycerol, 20 mM NaCl, pH: 7.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Jun 27 RgGuinier 3.8 nm Dmax 13.4 nm VolumePorod 179 nm3