The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity

Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
iScience 23(12):101811 (2020 Dec)

doi: 10.1016/j.isci.2020.101811

Submitted to SASBDB: 2019 Oct 25 Published in SASBDB: 2020 Nov 4

SASDGU9 – Nucleolar RNA helicase 2 (DDX21-FL) Sample: Nucleolar RNA helicase 2 dimer, 182 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 25 RgGuinier 7.0 nm Dmax 31.9 nm VolumePorod 681 nm3

SASDGV9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783 Sample: Nucleolar RNA helicase 2 fragment 186-783 dimer, 138 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 4 RgGuinier 4.8 nm Dmax 17.6 nm VolumePorod 297 nm3

SASDGW9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-710 Sample: Nucleolar RNA helicase 2 fragment 186-710 dimer, 121 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 4 RgGuinier 4.6 nm Dmax 17.7 nm VolumePorod 238 nm3

SASDGX9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-620 Sample: Nucleolar RNA helicase 2 fragment 186-620 dimer, 98 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 4 RgGuinier 4.4 nm Dmax 16.9 nm VolumePorod 150 nm3

SASDGY9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant Sample: Nucleolar RNA helicase 2 monomer, 69 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 3 RgGuinier 4.1 nm Dmax 17.7 nm VolumePorod 111 nm3