Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods.

Powers KT, Lavering ED, Washington MT
J Mol Biol 430(24):5294-5303 (2018 Dec 7)
PMID: 30381149
doi: 10.1016/j.jmb.2018.10.017 		Submitted to SASBDB: 2018 Oct 16
Published in SASBDB:

SASDED6 – Proliferating cell nuclear antigen - UbPCNA - Split Fusion Trimer

Proliferating cell nuclear antigen experimental SAS data
UIOWA_BD model
Sample: Proliferating cell nuclear antigen trimer, 117 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 238 nm3

SASDEE6 – Proliferating cell nuclear antigen - SUMOPCNA - Split Fusion Trimer

Proliferating cell nuclear antigen experimental SAS data
UIOWA_BD model
Sample: Proliferating cell nuclear antigen trimer, 125 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
RgGuinier 4.7 nm
Dmax 16.9 nm
VolumePorod 310 nm3