KAP1 is an antiparallel dimer with a functional asymmetry.

Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
Life Sci Alliance 2(4) (2019 Aug)

PMID: 31427381 doi: 10.26508/lsa.201900349

Submitted to SASBDB: 2018 Oct 23 Published in SASBDB: 2019 Aug 26

SASDEV6 – KRAB-associated protein 1 (KAP1); TRIM28; full length protein Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 dimer, 183 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6 RgGuinier 9.0 nm Dmax 38.0 nm VolumePorod 710 nm3

SASDER7 – KRAB-associated protein 1 (KAP1); TRIM28, amino acids 23-812 Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28; amino acids 23-812 dimer, 175 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6 RgGuinier 8.9 nm Dmax 38.0 nm VolumePorod 853 nm3

SASDEW6 – KRAB-associated protein 1, (KAP1); TRIM28; 23-418 RBCC domain Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28, Fragment 23-418, RBCC domain dimer, 92 kDa Homo sapiens protein Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6 RgGuinier 8.3 nm Dmax 37.0 nm VolumePorod 381 nm3