Structure of the Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain.

Viet KK, Wagner A, Schwickert K, Hellwig N, Brennich M, Bader N, Schirmeister T, Morgner N, Schindelin H, Hellmich UA
Structure (2019 May 14)

PMID: 31178222 doi: 10.1016/j.str.2019.04.016

Submitted to SASBDB: 2018 Oct 30 Published in SASBDB: 2019 Jun 11

SASDEC7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4 without Calcium Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein Buffer: 10 mM HEPES pH 7.4, 150 mM NaCl, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18 RgGuinier 3.4 nm Dmax 8.9 nm VolumePorod 134 nm3

SASDED7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 7.4, 2mM Calcium Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein Buffer: 10 mM HEPES pH 7.4, 150 mM NaCl, 2 mM CaCl2, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20 RgGuinier 3.4 nm Dmax 8.9 nm VolumePorod 134 nm3

SASDEE7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5, 0.5 mM Calcium Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein Buffer: 10 mM Hepes, pH 6.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20 RgGuinier 3.4 nm Dmax 8.9 nm VolumePorod 132 nm3

SASDEF7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 6.5 without Calcium Sample: Transient receptor potential channel mucolipin 2 tetramer, 93 kDa Homo sapiens protein Buffer: 10 mM HEPES pH 6.5 150 mM NaCl, pH: 6.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18 RgGuinier 3.4 nm Dmax 8.9 nm VolumePorod 140 nm3

SASDEG7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5 without Calcium Sample: Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein Buffer: 10 mM Hepes, pH 4.5, 150 mM NaCl, pH: 4.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 18 RgGuinier 3.7 nm Dmax 10.0 nm VolumePorod 285 nm3

SASDEH7 – Human TRPML2 Ion Channel Extracytosolic/Lumenal Domain at pH 4.5, 0.5 mM Calcium Sample: Transient receptor potential channel mucolipin 2 octamer, 187 kDa Homo sapiens protein Buffer: 10 mM Hepes, pH 4.5, 150 mM NaCl, 0.5 mM CaCl2, pH: 4.5 Experiment: SAXS data collected at BM29, ESRF on 2018 Oct 20 RgGuinier 3.7 nm Dmax 10.0 nm VolumePorod 280 nm3