A dual role in regulation and toxicity for the disordered N-terminus of the toxin GraT.

Talavera A, Tamman H, Ainelo A, Konijnenberg A, Hadži S, Sobott F, Garcia-Pino A, Hõrak R, Loris R
Nat Commun 10(1):972 (2019 Feb 27)

PMID: 30814507 doi: 10.1038/s41467-019-08865-z

Submitted to SASBDB: 2018 Nov 15 Published in SASBDB: 2019 Mar 4

SASDE48 – Toxin GraT Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2018 Sep 21 RgGuinier 1.5 nm Dmax 5.5 nm VolumePorod 18 nm3

SASDE58 – Complex of toxin GraT with antotoxin GraA Sample: Putative killer protein, Toxin GraT. monomer, 11 kDa Pseudomonas putida protein Antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein Putative killer protein, Toxin GraT monomer, 11 kDa Pseudomonas putida protein Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Jun 29 RgGuinier 2.3 nm Dmax 7.9 nm VolumePorod 63 nm3

SASDE68 – Two dimers of antitoxin GraA in complex with the grta operator Sample: GraTA operator region monomer, 20 kDa DNA Antitoxin GraA, antidote of toxin GraT dimer, 22 kDa Pseudomonas putida protein Atitoxin GraA, antidote of GraT. dimer, 23 kDa Pseudomonas putida protein Buffer: 50 mM Tris 250 mM NaCl 2 mM TCEP, pH: 8 Experiment: SAXS data collected at SWING, SOLEIL on 2017 Dec 17 RgGuinier 2.9 nm Dmax 10.3 nm VolumePorod 94 nm3