The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility.

Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
Nat Microbiol (2019 Dec 16)

PMID: 31844299 doi: 10.1038/s41564-019-0622-3

Submitted to SASBDB: 2018 Nov 12 Published in SASBDB: 2019 Nov 14

SASDEU7 – Stator protein FlaG soluble domain Sample: Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10 RgGuinier 3.7 nm Dmax 18.0 nm VolumePorod 133 nm3

SASDES7 – Stator protein complex FlaG/FlaF Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10 RgGuinier 3.2 nm Dmax 12.5 nm VolumePorod 109 nm3

SASDEV7 – Stator protein complex FlaG/FlaF-I96Y Sample: Stator protein FlaG soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein Conserved flagellar protein FlaF-I96Y soluble domain dimer, 33 kDa Sulfolobus acidocaldarius protein Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10 RgGuinier 2.7 nm Dmax 8.2 nm VolumePorod 90 nm3

SASDET7 – Stator protein complex FlaG-V118K/FlaF Sample: Conserved flagellar protein F dimer, 32 kDa Sulfolobus acidocaldarius protein Stator protein FlaG-V118K soluble domain dimer, 30 kDa Sulfolobus acidocaldarius protein Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10 RgGuinier 3.2 nm Dmax 12.5 nm VolumePorod 108 nm3