Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling.

Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X
Structure 27(8):1296-1307.e5 (2019 Aug 6)

PMID: 31257107 doi: 10.1016/j.str.2019.05.011

Submitted to SASBDB: 2018 Dec 11 Published in SASBDB: 2019 Sep 13

SASDE59 – Interleukin-1 receptor type 1, IL-1RI-ECD Sample: Interleukin-1 receptor type 1 monomer, 37 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 22 RgGuinier 3.0 nm Dmax 10.5 nm VolumePorod 63 nm3

SASDE69 – Interleukin-1 receptor type 2, IL-1RII-ECD Sample: Interleukin-1 receptor type 2 monomer, 38 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24 RgGuinier 2.8 nm Dmax 9.9 nm VolumePorod 80 nm3

SASDE79 – Interleukin-18 receptor 1, IL-18Rα-ECD Sample: Interleukin-18 receptor 1 monomer, 36 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2014 Oct 3 RgGuinier 3.1 nm Dmax 10.9 nm VolumePorod 77 nm3

SASDEZ8 – Interleukin-18 receptor accessory protein, IL-18Rβ-ECD Sample: Interleukin-18 receptor accessory protein monomer, 41 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24 RgGuinier 3.3 nm Dmax 11.5 nm VolumePorod 70 nm3

SASDE29 – Interleukin-1 receptor accessory protein ectodomain with RI linker Sample: Interleukin-1 receptor accessory protein ectodomain with RI linker monomer, 41 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Aug 11 RgGuinier 3.0 nm Dmax 10.4 nm VolumePorod 76 nm3

SASDE89 – Interleukin-1 receptor accessory protein ectodomain with RII linker Sample: Interleukin-1 receptor accessory protein ectodomains with RII linker monomer, 41 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24 RgGuinier 3.0 nm Dmax 10.7 nm VolumePorod 75 nm3

SASDE49 – Interleukin-1 receptor accessory protein ectodomains with ST2 linker Sample: Interleukin-1 receptor accessory protein ectodomains with ST2 linker monomer, 41 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24 RgGuinier 3.1 nm Dmax 10.7 nm VolumePorod 76 nm3

SASDE39 – Interleukin-18 receptor accessory protein ectodomain with Rα linker Sample: Interleukin-18 receptor accessory protein ectodomain with Rα linker monomer, 41 kDa Homo sapiens protein Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 24 RgGuinier 3.4 nm Dmax 11.5 nm VolumePorod 72 nm3