Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4.

Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM
Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015 Apr)

PMID: 25849406 doi: 10.1107/S1399004715002485

Submitted to SASBDB: 2014 Nov 12 Published in SASBDB: 2015 Jul 7

SASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains Sample: Integrin beta-4 monomer, 23 kDa Homo sapiens protein Buffer: 20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 6 RgGuinier 2.2 nm Dmax 7.0 nm VolumePorod 32 nm3