Structural Insights into the Intracellular Region of the Human Magnesium Transport Mediator CNNM4.

Giménez-Mascarell P, Oyenarte I, González-Recio I, Fernández-Rodríguez C, Corral-Rodríguez MÁ, Campos-Zarraga I, Simón J, Kostantin E, Hardy S, Díaz Quintana A, Zubillaga Lizeaga M, Merino N, Diercks T, Blanco FJ, Díaz Moreno I, Martínez-Chantar ML, Tremblay ML, Müller D, Siliqi D, Martínez-Cruz LA
Int J Mol Sci 20(24) (2019 Dec 12)

PMID: 31842432 doi: 10.3390/ijms20246279

Submitted to SASBDB: 2018 Dec 6 Published in SASBDB: 2020 Jan 9

SASDEN8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with Phosphatase of Regenerating Liver-1 and MgATP Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein Protein tyrosine phosphatase type IVA 1 dimer, 40 kDa Mus musculus protein Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 17 RgGuinier 4.4 nm Dmax 18.3 nm VolumePorod 216 nm3

SASDEP8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with Phosphatase of Regenerating Liver-1 Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein Protein tyrosine phosphatase type IVA 1 dimer, 40 kDa Mus musculus protein Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Aug 2 RgGuinier 4.7 nm Dmax 18.5 nm VolumePorod 226 nm3

SASDEQ8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Aug 2 RgGuinier 4.0 nm Dmax 16.6 nm VolumePorod 156 nm3

SASDER8 – Cyclic nucleotide homology domain of the human Cyclin and CBS domain divalent metal cation transport mediator-4 Sample: CNNM4_cNMP dimer, 42 kDa Homo sapiens protein Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 7 RgGuinier 2.6 nm Dmax 9.7 nm VolumePorod 102 nm3

SASDES8 – Intracellular region of the human Cyclin and CBS domain divalent metal cation transport mediator-4 in complex with MgATP Sample: CNNM4_BAT-cNMP-Ctail dimer, 96 kDa Homo sapiens protein Buffer: HEPES buffer pH 7.4, 200 mM NaCl , 1mM DTT, pH: 7.4 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 17 RgGuinier 3.9 nm Dmax 14.3 nm VolumePorod 149 nm3