Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations.

Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
Int J Mol Sci 19(12) (2018 Dec 12)

PMID: 30545121 doi: 10.3390/ijms19124012

Submitted to SASBDB: 2018 Dec 6 Published in SASBDB: 2018 Dec 14

SASDEW8 – Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3 Sample: Ribosome maturation protein SDO1 monomer, 17 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH: Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 14 RgGuinier 2.1 nm Dmax 6.2 nm VolumePorod 25 nm3

SASDEV8 – Shwachman-Bodian-Diamond Syndrome protein (yeast SDO1) Sample: Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21 RgGuinier 2.7 nm Dmax 8.5 nm

SASDEU8 – GTPase Elongation Factor like-1 protein (yeast EFL1) Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21 RgGuinier 4.7 nm Dmax 15.8 nm VolumePorod 258 nm3

SASDET8 – GTPase Elongation Factor like-1 bound Shwachman-Diamond Syndrome protein (EFL1*SDo1) Sample: Ribosome assembly protein 1 monomer, 124 kDa Saccharomyces cerevisiae protein Ribosome maturation protein SDO1 monomer, 28 kDa Saccharomyces cerevisiae protein Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH: Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 21 RgGuinier 5.0 nm Dmax 16.0 nm VolumePorod 333 nm3