Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly.

Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A
Nat Commun 1:95 (2010 Oct 19)

PMID: 20981023 doi: 10.1038/ncomms1097

Published in SASBDB: 2015 Jul 7

SASDAV6 – Cysteine desulfurase IscS dimer Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5 RgGuinier 3.2 nm Dmax 10.9 nm

SASDAW6 – Iron-sulfur cluster assembly scaffold IscU monomer Sample: Iron-sulfur cluster assembly scaffold protein IscU monomer, 14 kDa Escherichia coli protein Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5 RgGuinier 1.9 nm Dmax 6.4 nm

SASDAX6 – Protein CyaY monomer Sample: Protein CyaY monomer, 12 kDa Escherichia coli protein Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5 RgGuinier 1.5 nm Dmax 5.0 nm

SASDAY6 – IcsS-dimer and IscU-dimer complex Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5 RgGuinier 3.6 nm Dmax 12.1 nm

SASDAZ6 – CyaY-dimer and IscS-dimer complex Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Protein CyaY dimer, 24 kDa Escherichia coli protein Buffer: 20 mM Tris-HCl, 50 mM NaCl, 10 mM β-mercaptoethanol and ferrous ammonium sulphate, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5 RgGuinier 3.1 nm Dmax 10.9 nm

SASDA27 – IcsS, IscU and CyaY dimeric complex Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein Protein CyaY dimer, 24 kDa Escherichia coli protein Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8 Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Nov 5 RgGuinier 3.5 nm Dmax 11.9 nm