Identification of binding sites on human serum albumin for somapacitan - a long-acting growth hormone derivative.

Johansson E, Nielsen AD, Demuth H, Wiberg C, Schjødt C, Huang T, Chen J, Jensen S, Petersen J, Thygesen P
Biochemistry (2020 Mar 25)
PMID: 32208682
doi: 10.1021/acs.biochem.0c00019 		Submitted to SASBDB: 2019 Jan 28
Published in SASBDB:

SASDF22 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the human growth hormone derivative somapacitan.

somapacitanFcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagneonatal Fc receptor ectodomain alpha-chain experimental SAS data
somapacitan FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: Somapacitan monomer, 22 kDa Homo sapiens protein
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 May 11
RgGuinier 4.2 nm
Dmax 14.7 nm
VolumePorod 227 nm3

SASDF32 – Ternary complex of FcRn ectodomain, FcRn binding optimised human serum albumin and the albumin-binding side chain of the human growth hormone derivative somapacitan.

FcRn binding optimised human serum albumin V418M, T420A, E505G, V547Aneonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tagAlbumin-binding side-chainneonatal Fc receptor ectodomain alpha-chain experimental SAS data
FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag Albumin-binding side-chain neonatal Fc receptor ectodomain alpha-chain Kratky plot
Sample: FcRn binding optimised human serum albumin V418M, T420A, E505G, V547A monomer, 66 kDa Homo sapiens protein
Neonatal Fc receptor ectodomain beta-microglogulin part with C-terminal His6 tag monomer, 13 kDa Homo sapiens protein
Albumin-binding side-chain monomer, 1 kDa
Neonatal Fc receptor ectodomain alpha-chain monomer, 30 kDa Homo sapiens protein
Buffer: 100 mM MES, 100 mM NaCl, pH: 6.5
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Nov 11
RgGuinier 3.6 nm
Dmax 12.6 nm
VolumePorod 174 nm3

SASDF42 – Human serum albumin in complex with somapacitan

Human serum albuminSomapacitan experimental SAS data
SASREF model
Sample: Human serum albumin monomer, 66 kDa Homo sapiens protein
Somapacitan dimer, 44 kDa Homo sapiens protein
Buffer: 100 mM MES, 140 mM NaCl, pH: 6.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Novo Nordisk A/S on 2015 Sep 4
RgGuinier 4.1 nm
Dmax 13.9 nm
VolumePorod 202 nm3