A Tail-Based Mechanism Drives Nucleosome Demethylation by the LSD2/NPAC Multimeric Complex.

Marabelli C, Marrocco B, Pilotto S, Chittori S, Picaud S, Marchese S, Ciossani G, Forneris F, Filippakopoulos P, Schoehn G, Rhodes D, Subramaniam S, Mattevi A
Cell Rep 27(2):387-399.e7 (2019 Apr 9)

PMID: 30970244 doi: 10.1016/j.celrep.2019.03.061

Submitted to SASBDB: 2019 Mar 12 Published in SASBDB: 2019 Apr 16

SASDFU3 – Lysine-specific Demethylase (LSD2) Sample: Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11 RgGuinier 3.4 nm Dmax 10.6 nm VolumePorod 124 nm3

SASDFV3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH (NPAC delta-261) Sample: NPAC dehydrogenase domain tetramer, 125 kDa Homo sapiens protein Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3 Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 26 RgGuinier 3.5 nm Dmax 11.0 nm VolumePorod 167 nm3

SASDFW3 – Cytokine-like nuclear factor dehydrogenase domain, NPAC DH, plus native linker (NPAC delta-205) Sample: NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3 Experiment: SAXS data collected at BM29, ESRF on 2018 Jan 26 RgGuinier 4.0 nm Dmax 11.4 nm VolumePorod 240 nm3

SASDFX3 – Semi-synthetic nucleosome core particle (NCP) Sample: Histone H3 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 22 kDa Xenopus laevis protein Histone H2a dimer, 28 kDa Xenopus laevis protein Histone H2b dimer, 27 kDa Xenopus laevis protein 147bp 601 Widom sequence monomer, 45 kDa synthetic construct DNA Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11 RgGuinier 4.4 nm Dmax 12.8 nm VolumePorod 353 nm3

SASDFY3 – Semi-synthetic nucleosome core particle (NCP) in complex with lysine-specific demethylase (LSD2) and the dehydrogenase domain plus linker of cytokine-like nuclear factor (NPAC delta-205) Sample: Lysyne-specific Demethylase LSD2 monomer, 89 kDa Homo sapiens protein NPAC linker+DH (delta-205) tetramer, 150 kDa Homo sapiens protein Histone H3 dimer, 31 kDa Xenopus laevis protein Histone H4 dimer, 22 kDa Xenopus laevis protein Histone H2a dimer, 28 kDa Xenopus laevis protein Histone H2b dimer, 27 kDa Xenopus laevis protein 147bp 601 Widom sequence monomer, 45 kDa synthetic construct DNA Buffer: 15 mM HEPES, 200 mM NaCl, pH: 7.3 Experiment: SAXS data collected at BM29, ESRF on 2017 Dec 11 RgGuinier 7.8 nm Dmax 30.4 nm VolumePorod 1100 nm3