Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering.

Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S
FEBS J 282(8):1538-53 (2015 Apr)

PMID: 25702779 doi: 10.1111/febs.13240

Submitted to SASBDB: 2015 Jan 12 Published in SASBDB: 2015 Jul 7

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator Sample: Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein Response regulator dimer, 61 kDa Streptococcus pneumoniae protein Buffer: 20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5 Experiment: SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16 RgGuinier 4.0 nm Dmax 16.0 nm VolumePorod 175 nm3

SASDAB7 – Complex ComE-comcde Sample: Comcde, 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 61 kDa Streptococcus pneumoniae protein Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2 Experiment: SAXS data collected at SWING, SOLEIL on 2011 Feb 11 RgGuinier 3.4 nm Dmax 10.7 nm VolumePorod 122 nm3

SASDAC7 – Complex LytTR-comcde Sample: Comcde, 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 29 kDa Streptococcus pneumoniae protein Buffer: 50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2 Experiment: SAXS data collected at SWING, SOLEIL on 2013 Jun 2 RgGuinier 3.6 nm Dmax 12.5 nm VolumePorod 74 nm3