The quaternary structure of insulin glargine and glulisine under formulation conditions.

Nagel N, Graewert MA, Gao M, Heyse W, Jeffries CM, Svergun D, Berchtold H
Biophys Chem 253:106226 (2019 Oct)

PMID: 31376619 doi: 10.1016/j.bpc.2019.106226

Submitted to SASBDB: 2019 Mar 20 Published in SASBDB: 2019 Jul 26

SASDF94 – Insulin glulisine (Apidra), oligomeric composition Sample: Insulin glulisine hexamer, 35 kDa protein Buffer: Apidra formulation (per ml: 5 mg Sodium chloride, 3.15 mg m-Cresol, 6 mg Trometamol, 0.01 mg Polysorbate 20), pH: 7.3 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Apr 20 RgGuinier 2.3 nm Dmax 7.6 nm

SASDFA4 – Insulin glargine (Toujeo®), oligomeric composition Sample: Insulin glargine (Toujeo®) hexamer, 36 kDa protein Buffer: Toujeo Fromulation (190 ug Zinc chloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Jul 5 RgGuinier 1.8 nm Dmax 6.2 nm

SASDFB4 – Insulin glargine (Lantus ®), oligomeric mixture Sample: Insulin glargine (Lantus ®) hexamer, 36 kDa protein Buffer: Lantus Formulation (30 µg Zinc cloride, 2.7 mg m-Cresol, 20 mg glycerol 85%), pH: 4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 23 RgGuinier 1.8 nm Dmax 5.3 nm