Structures of three MORN repeat proteins and a re-evaluation of the proposed lipid-binding properties of MORN repeats.

Sajko S, Grishkovskaya I, Kostan J, Graewert M, Setiawan K, Trübestein L, Niedermüller K, Gehin C, Sponga A, Puchinger M, Gavin AC, Leonard TA, Svergun DI, Smith TK, Morriswood B, Djinovic-Carugo K
PLoS One 15(12):e0242677 (2020)

PMID: 33296386 doi: 10.1371/journal.pone.0242677

Submitted to SASBDB: 2019 Oct 1 Published in SASBDB: 2020 Jan 23

SASDG97 – Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 2-15 Sample: Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN repeats 2-15 dimer, 78 kDa Trypanosoma brucei protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5 Experiment: SAXS data collected at BM29, ESRF on 2017 Jan 27 RgGuinier 6.5 nm Dmax 26.0 nm VolumePorod 187 nm3

SASDGA7 – Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN1 repeats 7-15 Sample: Trypanosoma brucei Membrane Occupation and Recognition Nexus MORN (7-15) dimer, 52 kDa Trypanosoma brucei protein Buffer: 20 mM Tris-HCl, 200 mM NaCl, 2% (w/v) glycerol, 0.5 mM DTT, pH: 8.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11 RgGuinier 4.1 nm Dmax 15.5 nm VolumePorod 56 nm3

SASDGB7 – Toxoplasma gondii Membrane Occupation and Recognition Nexus (MORN1) repeats 7-15 Sample: Toxoplasma gondii Membrane Occupation and Recognition Nexus (MORN1) repeats 7-15 dimer, 49 kDa Toxoplasma gondii protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 10 RgGuinier 4.0 nm Dmax 17.0 nm VolumePorod 55 nm3

SASDGC7 – Plasmodium falciparum Membrane Occupation and Recognition Nexus (MORN1) repeats 7-15 Sample: MORN repeat-containing protein 1 dimer, 46 kDa Plasmodium falciparum protein Buffer: 20 mM Tris-HCl, 100 mM NaCl, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Dec 2 RgGuinier 3.8 nm Dmax 16.0 nm VolumePorod 52 nm3