Deciphering cellular and molecular determinants of human DPCD protein in complex with RUVBL1/RUVBL2 AAA-ATPases.

Dos Santos Morais R, Santo PE, Ley M, Schelcher C, Abel Y, Plassart L, Deslignière E, Chagot ME, Quinternet M, Paiva ACF, Hessmann S, Morellet N, M F Sousa P, Vandermoere F, Bertrand E, Charpentier B, Bandeiras TM, Plisson-Chastang C, Verheggen C, Cianférani S, Manival X
J Mol Biol :167760 (2022 Jul 25)

PMID: 35901867 doi: 10.1016/j.jmb.2022.167760

Submitted to SASBDB: 2019 Apr 15 Published in SASBDB: 2022 Aug 8

SASDFF5 – Protein DPCD Sample: Protein DPCD monomer, 24 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21 RgGuinier 2.2 nm Dmax 12.3 nm VolumePorod 39 nm3

SASDFH5 – DPCD His-RuvBl1/RuvBl2 hexamer complex Sample: Protein DPCD trimer, 71 kDa Homo sapiens protein RuvB-like 1 trimer, 155 kDa Homo sapiens protein RuvB-like 2 trimer, 156 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21 RgGuinier 5.4 nm Dmax 15.9 nm VolumePorod 856 nm3

SASDFG5 – His-RuvBl1/RuvBl2 dodecamer Sample: RuvB-like 1 hexamer, 311 kDa Homo sapiens protein RuvB-like 2 hexamer, 311 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 1% glycerol, 5 mM TCEP, pH: 7.5 Experiment: SAXS data collected at SWING, SOLEIL on 2019 Mar 21 RgGuinier 6.2 nm Dmax 20.0 nm VolumePorod 1490 nm3