Structural Insights Into the Transcriptional Regulation of HigBA Toxin–Antitoxin System by Antitoxin HigA in Pseudomonas aeruginosa

Liu Y, Gao Z, Liu G, Geng Z, Dong Y, Zhang H
Frontiers in Microbiology 10 (2020 Jan 22)

doi: 10.3389/fmicb.2019.03158 		Submitted to SASBDB: 2019 Apr 4
Published in SASBDB:

SASDF85 – Pseudomonas aeruginosa antitoxin HigA: apo PaHigA

Uncharacterized protein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Dec 21
RgGuinier 2.0 nm
Dmax 6.6 nm
VolumePorod 23 nm3

SASDF95 – Pseudomonas aeruginosa antitoxin HigA bound to duplex DNA: PaHigA-DNA complex

Uncharacterized proteinDNA Duplex experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uncharacterized protein dimer, 22 kDa Pseudomonas aeruginosa protein
DNA Duplex dimer, 20 kDa DNA
Buffer: 20 mM Tris, 300 mM NaCl, 5% (v/v) glycerol, and 1 mM PMSF, pH: 8
Experiment: SAXS data collected at BL19U2, Shanghai Synchrotron Radiation Facility (SSRF) on 2018 Sep 19
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 81 nm3