Nucleoid remodeling during environmental adaptation is regulated by HU-dependent DNA bundling (supplementary)

Soumya G Remesh

Submitted to SASBDB: 2019 Jun 4 Published in SASBDB: 2020 Apr 21

SASDFV6 – DNA-binding protein HU-alpha, E38K/V42L double mutant Sample: DNA-binding protein HU-alpha, E38K/V42L double mutant decamer, 95 kDa Escherichia coli protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23 RgGuinier 3.0 nm Dmax 10.5 nm VolumePorod 53 nm3

SASDFW6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:2) Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23 RgGuinier 5.7 nm Dmax 31.3 nm VolumePorod 297 nm3

SASDFX6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:4) Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant tetramer, 38 kDa Escherichia coli protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23 RgGuinier 5.7 nm Dmax 25.7 nm VolumePorod 195 nm3

SASDFY6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:8) Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant octamer, 76 kDa Escherichia coli protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23 RgGuinier 5.8 nm Dmax 28.1 nm VolumePorod 296 nm3

SASDFZ6 – DNA-binding protein HU-alpha, E38K/V42L double mutant bound to 80 bp DNA (ratio DNA:Protein 1:16) Sample: 80bp_DNA Forward monomer, 25 kDa Escherichia coli DNA 80bp_DNA Reverse monomer, 25 kDa Escherichia coli DNA DNA-binding protein HU-alpha, E38K/V42L double mutant 16-mer, 153 kDa Linked to wild-type … protein Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM DTT, 1 mM PMSF, pH: 7.5 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2015 Apr 23 RgGuinier 6.3 nm Dmax 27.3 nm VolumePorod 401 nm3