Studying Conformational Changes of the Yersinia Type-III-Secretion Effector YopO in Solution by Integrative Structural Biology.

Peter MF, Tuukkanen AT, Heubach CA, Selsam A, Duthie FG, Svergun DI, Schiemann O, Hagelueken G
Structure 27(9):1416-1426.e3 (2019 Sep 3)
PMID: 31303480
doi: 10.1016/j.str.2019.06.007 		Submitted to SASBDB: 2019 May 6
Published in SASBDB:

SASDFC6 – Wild type protein kinase YopO

Protein kinase YopO experimental SAS data
CUSTOM IN-HOUSE model
Sample: Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 6
RgGuinier 3.3 nm
Dmax 11.5 nm
VolumePorod 119 nm3

SASDFD6 – Protein kinase YopO - cytoplasmic 1 actin complex

Protein kinase YopOActin, cytoplasmic 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Protein kinase YopO monomer, 63 kDa Yersinia enterocolitica protein
Actin, cytoplasmic 1 monomer, 42 kDa Homo sapiens protein
Buffer: 10 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 6
RgGuinier 3.6 nm
Dmax 12.3 nm
VolumePorod 147 nm3