Tetramerization at low pH licenses DNA methylation activity of M.HpyAXI in the presence of acid stress.
Narayanan N,
Banerjee A,
Jain D,
Kulkarni DS,
Sharma R,
Nirwal S,
Rao DN,
Nair DT
J Mol Biol
(2019 Oct 16)
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Sample: |
Adenine specific DNA methyltransferase (Mod) dimer, 137 kDa Helicobacter pylori protein
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Buffer: |
25 mM Tris, 250 mM NaCl, pH: 8 |
Experiment: |
SAXS
data collected at ID14-3, ESRF on 2017 Jul 9
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RgGuinier |
3.3 |
nm |
Dmax |
12.5 |
nm |
VolumePorod |
143 |
nm3 |
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Sample: |
Adenine specific DNA methyltransferase (Mod) tetramer, 273 kDa Helicobacter pylori protein
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Buffer: |
25 mM citrate, 250 mM NaCl, pH: 5.5 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2018 Dec 1
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RgGuinier |
5.0 |
nm |
Dmax |
19.1 |
nm |
VolumePorod |
316 |
nm3 |
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