Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.

Garg A, Orru R, Ye W, Distler U, Chojnacki JE, Köhn M, Tenzer S, Sönnichsen C, Wolf E
J Biol Chem (2019 Sep 12)

PMID: 31515273 doi: 10.1074/jbc.RA119.009845

Submitted to SASBDB: 2019 Jun 5 Published in SASBDB: 2019 Sep 12

SASDF27 – Brain and Muscle ARNT-Like 1 from Mus musculus (D530-L625), monomer, trans-conformation locking mutation P624A Sample: Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein Buffer: 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27 RgGuinier 2.8 nm Dmax 11.0 nm

SASDF37 – KIX domain (kinase-inducible domain interacting domain) of CREB-binding protein (Mus musculus), mutation L664C Sample: Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein Buffer: 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27 RgGuinier 1.9 nm Dmax 8.1 nm

SASDF47 – Complex of CBP KIX domain with BMAL1 (D530-L625) including C-terminal transactivation domain (TAD) (Mus musculus) Sample: Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein Buffer: 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27 RgGuinier 2.8 nm Dmax 11.5 nm

SASDF57 – Complex of CBP KIX domain with BMAL1 (G517-L625) including C-terminal transactivation domain (TAD) (Mus musculus) Sample: Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) monomer, 11 kDa Mus musculus protein Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) monomer, 10 kDa Mus musculus protein Buffer: 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27 RgGuinier 3.1 nm Dmax 13.7 nm

SASDF67 – Complex of CBP KIX domain with BMAL1 (G490-L625) including C-terminal transactivation domain (TAD) (Mus musculus) Sample: Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) monomer, 10 kDa Mus musculus protein Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) monomer, 14 kDa Mus musculus protein Buffer: 25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27 RgGuinier 3.8 nm Dmax 17.5 nm

SASDF77 – Kinase-inducible domain interacting (KIX) domain of CREB-binding protein tethered to 1-10 Sample: Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein 1-{4-[4-chloro-3-(trifluoromethyl)phenyl]-4-hydroxypiperidin-1-yl}-3-sulfanylpropan-1-one monomer, 0 kDa Buffer: 25 mM Hepes, 150 NaCl, 5% Glycerol, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 May 27 RgGuinier 1.7 nm Dmax 6.4 nm