Structural and mechanistic insights into the interaction of the circadian transcription factor BMAL1 with the KIX domain of the CREB-binding protein.
Garg A,
Orru R,
Ye W,
Distler U,
Chojnacki JE,
Köhn M,
Tenzer S,
Sönnichsen C,
Wolf E
J Biol Chem
(2019 Sep 12)
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Sample: |
Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein
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Buffer: |
25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 May 27
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RgGuinier |
2.8 |
nm |
Dmax |
11.0 |
nm |
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Sample: |
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein
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Buffer: |
25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 May 27
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RgGuinier |
1.9 |
nm |
Dmax |
8.1 |
nm |
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Sample: |
Aryl hydrocarbon receptor nuclear translocator-like protein 1 monomer, 10 kDa Mus musculus protein
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein
|
Buffer: |
25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 May 27
|
|
RgGuinier |
2.8 |
nm |
Dmax |
11.5 |
nm |
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Sample: |
Brain and muscle ARNT-like 1 (G517-L625) including transactivation domain (TAD) monomer, 11 kDa Mus musculus protein
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) monomer, 10 kDa Mus musculus protein
|
Buffer: |
25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 May 27
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|
RgGuinier |
3.1 |
nm |
Dmax |
13.7 |
nm |
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Sample: |
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP) monomer, 10 kDa Mus musculus protein
Brain and muscle ARNT-like 1 (G490-L625) including transactivation domain (TAD) monomer, 14 kDa Mus musculus protein
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Buffer: |
25 mM Hepes, 150 NaCl, 1 mM DTT, 5% Glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 May 27
|
|
RgGuinier |
3.8 |
nm |
Dmax |
17.5 |
nm |
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Sample: |
Kinase-inducible domain interacting (KIX) domain of CREB-binding protein (CBP), mutation L664C monomer, 10 kDa Mus musculus protein
1-{4-[4-chloro-3-(trifluoromethyl)phenyl]-4-hydroxypiperidin-1-yl}-3-sulfanylpropan-1-one monomer, 0 kDa
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Buffer: |
25 mM Hepes, 150 NaCl, 5% Glycerol, pH: 7.2 |
Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2017 May 27
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|
RgGuinier |
1.7 |
nm |
Dmax |
6.4 |
nm |
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