The F1 loop of the talin head domain acts as a gatekeeper in integrin activation and clustering.

Kukkurainen S, Azizi L, Zhang P, Jacquier MC, Baikoghli M, von Essen M, Tuukkanen A, Laitaoja M, Liu X, Rahikainen R, Orłowski A, Jänis J, Määttä JAE, Varjosalo M, Vattulainen I, Róg T, Svergun D, Cheng RH, Wu J, Hytönen VP, Wehrle-Haller B
J Cell Sci 133(19) (2020 Oct 12)

PMID: 33046605 doi: 10.1242/jcs.239202

Submitted to SASBDB: 2019 Jun 23 Published in SASBDB: 2020 Sep 22

SASDFS7 – Talin-1 head amino acids 1-405(Δ139-168) Sample: Talin-1 (Δ139-168), human monomer, 48 kDa Homo sapiens protein Buffer: 50 mM sodium phosphate, 150mM NaCl, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24 RgGuinier 3.3 nm Dmax 13.3 nm VolumePorod 77 nm3

SASDFT7 – Talin-1 head amino acids 1-405 Sample: Talin-1, human monomer, 51 kDa Homo sapiens protein Buffer: 50 mM sodium phosphate, 150 mM NaCl, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24 RgGuinier 3.4 nm Dmax 11.5 nm VolumePorod 94 nm3

SASDFU7 – Talin-1 head amino acids 1-405(Δ134-170/GAG insert) Sample: Talin-1 (Δ134-170/GAG insert), human monomer, 47 kDa Homo sapiens protein Buffer: 50 mM sodium phosphate, 150mM NaCl, pH: 7.2 Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24 RgGuinier 3.0 nm Dmax 10.2 nm VolumePorod 71 nm3