Flexible Tethering of ASPP Proteins Facilitates PP-1c Catalysis.

Zhou Y, Millott R, Kim HJ, Peng S, Edwards RA, Skene-Arnold T, Hammel M, Lees-Miller SP, Tainer JA, Holmes CFB, Glover JNM
Structure 27(10):1485-1496.e4 (2019 Oct 1)

PMID: 31402222 doi: 10.1016/j.str.2019.07.012

Submitted to SASBDB: 2019 Oct 2 Published in SASBDB: 2019 Nov 1

SASDG76 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact Sample: Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 31 RgGuinier 3.1 nm Dmax 11.3 nm VolumePorod 157 nm3

SASDG86 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(608-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended Sample: Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2018 May 31 RgGuinier 3.4 nm Dmax 12.2 nm VolumePorod 180 nm3

SASDG66 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25 RgGuinier 3.0 nm Dmax 10.9 nm VolumePorod 116 nm3

SASDG56 – Inhibitor of apoptosis-stimulating protein of p53 (iASPP(621-828)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein Inhibitor of apoptosis-stimulating protein of p53 (RelA-associated inhibitor) monomer, 25 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Apr 25 RgGuinier 3.4 nm Dmax 12.4 nm VolumePorod 132 nm3

SASDG96 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, compact Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein Apoptosis-stimulating of p53 protein 2 monomer, 26 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 31 RgGuinier 2.9 nm Dmax 10.2 nm VolumePorod 144 nm3

SASDGA6 – Apoptosis-stimulating protein 2 of p53 (ASPP2(905-1128)) bound to the serine/threonine-protein phosphatase PP1-alpha catalytic subunit, extended Sample: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit monomer, 38 kDa Homo sapiens protein Apoptosis-stimulating of p53 protein 2 monomer, 26 kDa Homo sapiens protein Buffer: 25 mM Tris, 150 mM NaCl, 1 mM DTT, pH: 8 Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 31 RgGuinier 3.3 nm Dmax 11.7 nm VolumePorod 172 nm3