Structural and Mechanistic Insights into Caffeine Degradation by the Bacterial N-Demethylase Complex.

Kim JH, Kim BH, Brooks S, Kang SY, Summers RM, Song HK
J Mol Biol 431(19):3647-3661 (2019 Sep 6)

PMID: 31412262 doi: 10.1016/j.jmb.2019.08.004

Submitted to SASBDB: 2019 Jun 17 Published in SASBDB: 2019 Sep 16

SASDFD7 – Pseudomonas putida CBB5 NdmAB complex - static Sample: Methylxanthine N1-demethylase NdmA trimer, 127 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27 RgGuinier 4.5 nm Dmax 12.3 nm

SASDFE7 – Pseudomonas putida CBB5 mCherry-NdmA/NdmB complex - static Sample: Methylxanthine N1-demethylase NdmA trimer, 207 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27 RgGuinier 5.4 nm Dmax 13.8 nm

SASDFF7 – Pseudomonas putida CBB5 NdmA hexamer Sample: Methylxanthine N1-demethylase NdmA hexamer, 254 kDa Pseudomonas putida protein Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 May 21 RgGuinier 4.2 nm Dmax 11.0 nm

SASDFG7 – Pseudomonas putida CBB5 NdmB hexamer Sample: Methylxanthine N3-demethylase NdmB hexamer, 258 kDa Pseudomonas putida protein Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 May 21 RgGuinier 4.3 nm Dmax 12.2 nm

SASDFH7 – Pseudomonas putida CBB5 NdmAB complex Sample: Methylxanthine N1-demethylase NdmA trimer, 127 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 129 kDa Pseudomonas putida protein Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP, pH: 7.5 Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2018 May 21 RgGuinier 4.2 nm Dmax 10.9 nm

SASDFJ7 – Pseudomonas putida CBB5 mCherry-NdmA/ECFP-NdmB complex - static Sample: Methylxanthine N1-demethylase NdmA trimer, 207 kDa Pseudomonas putida protein Methylxanthine N3-demethylase NdmB trimer, 208 kDa Pseudomonas putida protein Buffer: 20 mM HEPES 150 mM NaCl 2 mM TCEP 10% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at 4C, Pohang Accelerator Laboratory on 2018 Jul 27 RgGuinier 5.6 nm Dmax 19.1 nm