The Methionine-Rich Loop of Multicopper Oxidase McoA follows Open-To-Close Transitions with a Role in Enzyme Catalysis
Borges P,
Brissos V,
Hernandez G,
Masgrau L,
Lucas M,
Monza E,
Frazão C,
Cordeiro T,
Martins L
ACS Catalysis
(2020 Jun 02)
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Sample: |
Aquifex aeolicus McoA metaloxidase monomer, 55 kDa Aquifex aeolicus protein
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Buffer: |
50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2019 Apr 15
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RgGuinier |
2.3 |
nm |
Dmax |
7.5 |
nm |
VolumePorod |
79 |
nm3 |
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Sample: |
Aquifex aeolicus McoA metaloxidase ∆337-346 monomer, 54 kDa Aquifex aeolicus protein
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Buffer: |
50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5 |
Experiment: |
SAXS
data collected at B21, Diamond Light Source on 2017 Dec 4
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RgGuinier |
2.3 |
nm |
Dmax |
7.0 |
nm |
VolumePorod |
78 |
nm3 |
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Sample: |
Aquifex aeolicus McoA metaloxidase ∆328-352 (MCoA∆328-352) monomer, 53 kDa Aquifex aeolicus protein
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Buffer: |
50 mM Tris-HCl, 150 mM NaCl, 2 mM TCEP, pH: 7.5 |
Experiment: |
SAXS
data collected at BM29, ESRF on 2017 Jul 13
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RgGuinier |
2.3 |
nm |
Dmax |
6.9 |
nm |
VolumePorod |
77 |
nm3 |
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