Molecular basis of F-actin regulation and sarcomere assembly via myotilin

Kostan J, Pavšič M, Puž V, Schwarz T, Drepper F, Molt S, Graewert M, Schreiner C, Sajko S, van der Ven P, Onipe A, Svergun D, Warscheid B, Konrat R, Fürst D, Lenarčič B, Djinović-Carugo K, Machesky L
PLOS Biology 19(4):e3001148 (2021 Apr 12)

doi: 10.1371/journal.pbio.3001148

Submitted to SASBDB: 2019 Jun 28 Published in SASBDB: 2020 Oct 28

SASDFZ7 – Full-length myotilin with N-terminally fused thioredoxin tag Sample: His-trx-myotilin monomer, 70 kDa Homo sapiens protein Buffer: 20 mM Tris, 400 mM NaCl, 250 mM arginine, 5% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Jun 11 RgGuinier 5.1 nm Dmax 16.7 nm VolumePorod 131 nm3

SASDF28 – Myotilin immunoglobulin domains Ig1Ig2 (220-452) Sample: Myotilin Ig1Ig2 (220-452) monomer, 27 kDa Homo sapiens protein Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2010 Nov 16 RgGuinier 3.7 nm Dmax 15.1 nm VolumePorod 52 nm3

SASDF38 – Myotilin immunoglobulin domains Ig1Ig2 (250-444) Sample: Myotilin Ig1Ig2 (250-444) monomer, 22 kDa Homo sapiens protein Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2019 Jun 27 RgGuinier 2.8 nm Dmax 10.1 nm VolumePorod 29 nm3

SASDF48 – Myotilin immunoglobulin domains Ig1Ig2 (250-498) Sample: Myotilin Ig1Ig2 (250-498) monomer, 28 kDa Homo sapiens protein Buffer: 20 mM Na+-HEPES, 150 mM, NaCl, 5 % v/v glycerol, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at BM29, ESRF on 2019 Jun 27 RgGuinier 3.2 nm Dmax 14.0 nm VolumePorod 37 nm3

SASDJH8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, wild-type) concentration series data Sample: Myotilin (222-452) monomer, 26 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 6 RgGuinier 3.3 nm Dmax 11.5 nm VolumePorod 36 nm3

SASDJJ8 – Myotilin immunoglobulin domains Ig1Ig2 (220-452, R405K mutant) concentration series data Sample: Myotilin (222-452) R405K monomer, 26 kDa Homo sapiens protein Buffer: 20 mM HEPES, 150 mM NaCl, 5 % glycerol, 1 mM DTT, pH: 7.4 Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jul 12 RgGuinier 3.3 nm Dmax 10.5 nm VolumePorod 36 nm3