Staufen1 reads out structure and sequence features in ARF1 dsRNA for target recognition.

Yadav DK, Zigáčková D, Zlobina M, Klumpler T, Beaumont C, Kubíčková M, Vaňáčová Š, Lukavsky PJ
Nucleic Acids Res (2019 Dec 25)

PMID: 31875226 doi: 10.1093/nar/gkz1163

Submitted to SASBDB: 2019 Jul 8 Published in SASBDB: 2019 Nov 29

SASDF29 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 Sample: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 4 monomer, 8 kDa Homo sapiens protein ADP-ribosylation factor1 - short monomer, 11 kDa Homo sapiens RNA Buffer: 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH: 6.8 Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2015 Dec 10 RgGuinier 1.7 nm Dmax 6.0 nm VolumePorod 21 nm3

SASDF39 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 Sample: ADP-ribosylation factor1 - short monomer, 11 kDa Homo sapiens RNA Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 monomer, 20 kDa Homo sapiens protein Buffer: 50 mM sodium phosphate buffer, 300 mM NaCl, 500 mM imidazole, pH: 8 Experiment: SAXS data collected at Rigaku BioSAXS-1000, CEITEC on 2018 May 29 RgGuinier 2.1 nm Dmax 6.4 nm VolumePorod 39 nm3

SASDF49 – Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 - in complex with STAU1 binding site within the ADP-ribosylation factor1 - long Sample: Double-stranded RNA-binding protein Staufen homolog 1 - RNA binding domain 3 and 4 monomer, 20 kDa Homo sapiens protein ADP-ribosylation factor1 - long monomer, 16 kDa Homo sapiens RNA Buffer: 50 mM potassium phosphate, 100 mM NaCl, 3.5 mM 2-mercaptoethanol, pH: 6.8 Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Sep 20 RgGuinier 2.5 nm Dmax 7.0 nm VolumePorod 53 nm3