Quantitative Conformational Analysis of Functionally Important Electrostatic Interactions in the Intrinsically Disordered Region of Delta Subunit of Bacterial RNA Polymerase.

Kuban V, Srb P, Stegnerova H, Padrta P, Zachrdla M, Jasenakova Z, Šanderová H, Vítovská D, Krasny L, Koval T, Dohnalek J, Ziemska-Legi Cka J, Grynberg M, Jarnot P, Gruca A, Jensen MR, Blackledge M, Zidek L
J Am Chem Soc (2019 Sep 24)

PMID: 31550880 doi: 10.1021/jacs.9b07837

Submitted to SASBDB: 2019 Jun 22 Published in SASBDB: 2019 Sep 30

SASDF58 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 10mM NaCl Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 3.5 nm Dmax 14.0 nm VolumePorod 38 nm3

SASDF68 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 200 mM NaCl Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 3.9 nm Dmax 20.0 nm VolumePorod 56 nm3

SASDF78 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 400 mM NaCl Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.2 nm Dmax 20.0 nm VolumePorod 63 nm3

SASDF88 – Delta subunit of RNA polymerase, RNAP (B. subtilis), 800 mM NaCl Sample: DNA-directed RNA polymerase subunit delta monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.5 nm Dmax 21.0 nm VolumePorod 77 nm3

SASDF98 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 10mM NaCl Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 10 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.3 nm Dmax 19.5 nm VolumePorod 58 nm3

SASDFA8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 200mM NaCl Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 200 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.6 nm Dmax 22.0 nm VolumePorod 76 nm3

SASDFB8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 400mM NaCl Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 400 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.5 nm Dmax 24.0 nm VolumePorod 78 nm3

SASDFC8 – Delta subunit of RNA polymerase, RNAP (B. subtilis): Lysine to glutamate mutant, 800mM NaCl Sample: DNA-directed RNA polymerase subunit delta - mutant monomer, 20 kDa Bacillus subtilis protein Buffer: 20 mM Phosphate buffer, 800 mM NaCl, 0.05% NaN3, pH: 6.6 Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 3 RgGuinier 4.5 nm Dmax 21.0 nm VolumePorod 74 nm3