Structural properties of [2Fe-2S] ISCA2-IBA57: a complex of the mitochondrial iron-sulfur cluster assembly machinery

Nasta V, Da Vela S, Gourdoupis S, Ciofi-Baffoni S, Svergun D, Banci L
Scientific Reports 9(1) (2019 Dec 12)

doi: 10.1038/s41598-019-55313-5 		Submitted to SASBDB: 2019 Jul 22
Published in SASBDB:

SASDGD2 – Putative mitochondrial transferase CAF17: human IBA57

Putative transferase CAF17, mitochondrial experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Jul 10
RgGuinier 2.2 nm
Dmax 7.1 nm
VolumePorod 50 nm3

SASDGE2 – Human mitochondrial iron-sulfur cluster assembly ISCA2 protein

Iron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
SASREF model
Sample: Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Sep 16
RgGuinier 2.0 nm
Dmax 6.7 nm
VolumePorod 31 nm3

SASDGF2 – Putative mitochondrial transferase CAF17 bound to mitochondrial iron-sulfur cluster assembly ISCA2 protein (human IBA57-ISCA2 complex)

Putative transferase CAF17, mitochondrialIron-sulfur cluster assembly 2 homolog, mitochondrial experimental SAS data
SASREF CV model
Sample: Putative transferase CAF17, mitochondrial monomer, 33 kDa Homo sapiens protein
Iron-sulfur cluster assembly 2 homolog, mitochondrial dimer, 24 kDa Homo sapiens protein
Buffer: 50 mM phosphate, 150 mM NaCl, 5 mM DTT, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 Nov 22
RgGuinier 4.0 nm
Dmax 16.4 nm
VolumePorod 114 nm3