High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE.

Azmi L, Bragginton EC, Cadby IT, Byron O, Roe AJ, Lovering AL, Gabrielsen M
Acta Crystallogr F Struct Biol Commun 76(Pt 9):414-421 (2020 Sep 1)

PMID: 32880589 doi: 10.1107/S2053230X20010237

Submitted to SASBDB: 2017 May 11 Published in SASBDB: 2017 Dec 12

SASDC72 – Alcohol dehydrogenase from bifunctional alcohol/aldehyde dehydrogenase (P0A9Q8) Sample: Aldehyde-alcohol dehydrogenase dimer, 97 kDa Escherichia coli O157:H7 protein Buffer: 20 mM Tris 400 mM NaCl 5% v/v glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2015 Nov 27 RgGuinier 3.2 nm Dmax 16.9 nm VolumePorod 146 nm3

SASDCK3 – Aldehyde dehydrogenase Sample: Aldehyde-alcohol dehydrogenase monomer, 48 kDa Escherichia coli O157:H7 protein Buffer: 30 mM HEPES, 150 mM NaCl, 5% (v/v) glycerol, pH: 7.5 Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Jan 30 RgGuinier 2.7 nm Dmax 11.1 nm VolumePorod 74 nm3